Table 1 Partial purification of AThTP-synthesizing enzyme from E. coli
Fraction | Volume (ml) | Total protein (mg) | Total activity (pmol.min-1) | Specific activity (pmol.min-1.mg-1) | Purification factor |
|---|---|---|---|---|---|
Extract | 32.0 | 99.2 | 436.5 | 4.4 | 1 |
Heat treatment | 31.0 | 71.3 | 370.8 | 5.2 | 1.2 |
Sephadex G-200: | |||||
Peak I (maximum) | 4.0 | 0.68 | 63.4 | 93.2 | 21.2 |
Peak II (maximum) | 4.0 | 0.94 | 75.4 | 80.2 | 18.2 |