Figure 3From: Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphateEffect of pH on AThTP synthesizing enzyme. The Mg2+ concentration was 10 mM, and the buffers used were as follows: (○) 50 mM acetate, pH 5.5; 50 mM maleate, pH 6.0–6.5; 50 mM Tris-maleate, pH 7.0; 50 mM Tris-HCl, pH 7.5; (■) 50 mM Bis-Tris-propane; (□) 100 mM Bis-Tris-propane.Back to article page