Figure 2From: Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphateTime dependence of AThTP synthesis. The experiment was carried out under standard conditions (1 mM ADP, 0.1 mM ThDP, 10 mM Mg2+, maleate buffer, pH 6.5), and the protein concentration was 100 μg/ml.Back to article page