Table 1 Oligonucleotides used in this study
From: C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complex
Oligo# | Sequence (5'-3') | Construct(s) |
|---|---|---|
4009-1 | GCGGCCGCTGGAAGAAAAGGATTGAATATGG | 3' fragment of SPT7 |
4009-2 | GAATTCTATTCAACTATTTAGCGCGCTC | Â |
4051-1 | GCGGCCGCAATGACTGAAAGAATACCAATAAAG | 5'fragment of SPT7 |
5051-2 | CCAAATCGTCTCTATCGTCATCC | Â |
4536-1 | GATGCGGCCGCGATGGAAGACGCTTCCGTG | 5' truncations of SPT7 |
4536-2 | GATCGGCCGCCGGTATCAACAGGCCAGAC | Â |
4213-1 | GGCCGACTACAAGGACGACGATGACAAGGC | N-terminal Flag epitope |
4213-2 | GGCCGCCTTGTCATCGTCGTCCTTGTAGTC | Â |
4149-4 | GAGCTCGCGGCCGCATAATCAAGTGCCCCGGAG | TAP-tag |
4168-1 | GGATCCATGAAAGCTGATGCGCAACAAATT | Â |
4356-1 | GATGCGGCCGCGATGGAAGACGCTTCGTG | DED1 promoter |
4356-2 | GATGCGGCCGCCGGTATCAACAGGCCAGAC | Â |
2764-1 | AACTGCAGTAATACGCTTAACTGCTC | GAL10 promoter |
2764-2 | CCCAAGCTTGACGTTAAAGTATAGAGGT | Â |
2821-1 | ACGAAGCTTACCATGGACTACAAGGACGACGAT | Met-Flag tag |
2821-2 | TTGAGCTCTGCGGCCGCCTTGTCATCGTCGTCC | Â |