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Table 3 Summary of 11-cis retinal binding parameters to IRBP. The binding of 11-cis retinal to full-length IRBP and the four modules was determined by competitive fluorescence spectroscopy using all-trans retinol.

From: Module structure of interphotoreceptor retinoid-binding protein (IRBP) may provide bases for its complex role in the visual cycle – structure/function study of Xenopus IRBP

Protein Binding sites (N) Kd (μM) All-trans retinol Kd (μM) 11-cis retinal
Full-length 3.53 ± 0.19 0.22 ± 0.13 0.21 ± 0.10
Module 1 1.29 ± 0.24 0.67 ± 0.17 0.57 ± 0.11
Module 2 1.22 ± 0.10 0.074 ± 0.037 0.28 ± 0.13
Module 3 1.90 ± 0.28 0.84 ± 0.22 0.56 ± 0.11
Module 4 1.47 ± 0.18 0.41 ± 0.09 0.29 ± 0.05