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Table 3 Summary of 11-cis retinal binding parameters to IRBP. The binding of 11-cis retinal to full-length IRBP and the four modules was determined by competitive fluorescence spectroscopy using all-trans retinol.

From: Module structure of interphotoreceptor retinoid-binding protein (IRBP) may provide bases for its complex role in the visual cycle – structure/function study of Xenopus IRBP

Protein

Binding sites (N)

Kd (μM) All-trans retinol

Kd (μM) 11-cis retinal

Full-length

3.53 ± 0.19

0.22 ± 0.13

0.21 ± 0.10

Module 1

1.29 ± 0.24

0.67 ± 0.17

0.57 ± 0.11

Module 2

1.22 ± 0.10

0.074 ± 0.037

0.28 ± 0.13

Module 3

1.90 ± 0.28

0.84 ± 0.22

0.56 ± 0.11

Module 4

1.47 ± 0.18

0.41 ± 0.09

0.29 ± 0.05

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BMC Biochemistry

ISSN: 1471-2091

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