Effect of polyamines and synthetic polyamine-analogues on the expression of antizyme (AtoC) and its regulatory genes

Table 3 Effect of polyamine analogues on ODC activity.

BW25113 strain/1 mM Substance	% ODC activity	% Inhibition of ODC activity	ODC Units/10 μl	Specific ODC activity (Units/mg protein)
Control	100	0	4.79	239.5 ± 3.5
PUT	40.66	59.33	1.95	84.78 ± 0.5
SPD	47.89	52.11	2.29	76.33 ± 0.2
SPM	76.94	23.06	3.68	153.33 ± 2.5
8	74.98	25.02	3.59	211.17 ± 4.0
10	51.78	48.22	2.48	118.09 ± 0.3
12	37.52	62.48	1.79	99.44 ± 1.5
13	61.36	38.64	2.94	108.88 ± 2.3
14	56.82	43.18	2.72	82.42 ± 0.2

E. coli BW25113 was grown to an OD₆₀₀ 0.8–0.9 in M9 medium in the absence (control) or in the presence of 1 mM polyamine (PUT, SPD, SPM) or 1 mM polyamine analogue. ODC activity and protein levels were determined as described in Materials and Methods. Specific activities are expressed as μmol ¹⁴CO₂ liberated/mg enzyme protein/h of incubation. The values represent the means ± SD from two separate experiments.