Table 2 Effect of polyamine analogues in the transcriptional activation of the atoC gene and atoDAEB operon in E. coli strain BW25113.
SUBSTANCES | % β-galactosidase activity BW25113/(atoC-lacZ) | % β-galactosidase activity BW25113/(atoA-lacZ) |
|---|---|---|
Control (No polyamines added) | 100% | 100% |
1 mM concentration | ||
SPERMINE ANALOGUES | ||
spermine | 98 ± 3.38 | 88 ± 1.98 |
1 | 98 ± 1.9 | 80 ± 5.96 |
2 | 156 ± 1.62 | 79 ± 1.59 |
3 | 80 ± 1.41 | 58 ± 2.49 |
4 | N.D | N.D |
SPERMIDINE ANALOGUES | ||
spermidine | 105 ± 1.38 | 107 ± 3.37 |
5 | 107 ± 1.82 | 60 ± 2.84 |
6 | 128 ± 1.73 | 52 ± 3.76 |
7 | N.D | N.D |
PUTRESCINE ANALOGUES | ||
putrescine | 170 ± 4.29 | 110 ± 3.28 |
8 | 125 ± 1.63 | 76 ± 1.93 |
9 | N.D | N.D |
10 | 216 ± 3.92 | 233 ± 6.30 |
11 | N.D | N.D |
DIAMINOPROPANE ANALOGUES | ||
diaminopropane | 200 ± 4.19 | 87 ± 2.26 |
12 | 238 ± 6.44 | 128 ± 5.47 |
13 | 185 ± 9.81 | 202 ± 9.68 |
14 | 303 ± 6.80 | 193 ± 7.35 |