Figure 7
Effect of polyamine analogues on endogenous ODC expression. E. coli K12 cells BW25113 (atoSC+) were grown in the presence of 1 mM of the indicated polyamine or polyamine analogue, to a cell density of 0.8–0.9. The total cell extracts from each culture (20 μl) were subjected to SDS-PAGE 10% and immunostained with anti-ODC. Lane 1: BW25113 cells, with no inducer added. Lanes 2–4: BW25113 cells, treated with 1 mM PUT or SPD or SPM. Lanes 5–9: BW25113 cells treated with 1 mM of each polyamine analogue 8, 10, 12, 13 and 14, respectively.