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Figure 3 | BMC Biochemistry

Figure 3

From: The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site

Figure 3

Kinetic analysis of SF3b1 phosphorylation by DYRK1A and CDK2. SF3b1-NT-His6 was phosphorylated with GST-DYRK1A-ΔC or cyclin E/CDK2 at two different substrate concentrations (0.7 and 7 μM). Phosphate incorporation was measured at different time points (2, 4 and 8 minutes). The slope of the straight line reflects the velocity of phosphorylation at the different substrate concentrations. The calculated K m values are indicated. The experiment was repeated with similar results.

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BMC Biochemistry

ISSN: 1471-2091

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