Figure 5

Western blots and TLC scanning results for v-Ki-Ras2 (K-Ras-4B) with radioactive prenyl anchor precursors. Western Blot and corresponding scans from TLC linear analyzer of wildtype GST-v-Ki-Ras2-fusion protein translated with [³H]mevalonic acid (lane 1), GST- v-Ki-Ras2 C185A with [³H]mevalonic acid (lane 2), GST-K-Ras with [³H]FPP (lane 3) and GST-K-Ras with [³H]GGPP (lane 4). There is incorporation of a product of mevalonic acid (lane 1) and FPP (lane 3) and also a reduced but noticeable amount of GGPP (lane 4), supporting the view of alternative geranylgeranylation of K-Ras in the absence of farnesylation.