Figure 1

Western blots and TLC scanning results for Rap2A with radioactive prenyl anchor precursors. Western Blot and corresponding scans from TLC linear analyzer of wildtype GST-Rap2A-fusion protein translated with [³H]mevalonic acid (lane 1), GST-Rap2A C180A with [³H]mevalonic acid (lane 2), GST-Rap2A with [³H]FPP (lane 3) and GST-Rap2A with [³H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid (lane 1) as well as FPP (lane 3), while incorporation of GGPP is close to the detection limit (lane 4), suggesting that Rap2A is primarily a farnesylation target.