Figure 5

Phosphorylation of MEF2C at S396 and sumoylation of MEF2C at K391 repress transcription through the same pathway. (A) Three possible models with respect to the relationship between phosphorylation and sumoylation of MEF2C. (B) GAL4×5-luciferase reporter and pRL-tk reporter constructs were co-transfected with various Gal4-MEF2C constructs into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities. (C) LexA×8-GAL4×5-luciferase reporter, pRL-tk reporter, and LexA-VP16 constructs were cotransfected with various Gal4-MEF2C constructs into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities.