Figure 4
From: Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity
A small C-terminal fragment of MEF2C is sufficient to repress transcription in a sumoylation-dependent manner. (A) Schematic drawing of the functional domains and two C-terminal fragments of MEF2C. (B) Gal4-MEF2C-WT, Gal4-MEF2C-K391R, Gal4-MEF2C-ΔN1, or Gal4-MEF2C-ΔN1-K391R construct was co-transfected with GAL4×5-luciferase reporter and pRL-tk reporter constructs into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities. (C) Gal4, Gal4-MEF2C-ΔN2, or Gal4-MEF2C-ΔN2-K391R construct was cotransfected with LexA×8-GAL4×5-luciferase reporter, pRL-tk reporter, and LexA-VP16 constructs into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities. (D) HeLa cells were transfected with the indicated plasmids. The total cell lysates were resolved by SDS-PAGE and blotted with anti-MEF2C. The asterisk indicates the endogenous MEF2 proteins.