Figure 3

Sumoylation of MEF2C inhibits its transcriptional activity. (A) Gal4, Gal4-MEF2C-WT, or Gal4-MEF2C-K391R construct was co-transfected with GAL4×5-luciferase reporter and pRL-tk reporter into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities. (B) Gal4-MEF2C-WT or Gal4-MEF2C-K391R construct was co-transfected with GAL4×5-luciferase reporter, pRL-tk reporter, and SENP2, DN-UBC9-Flag, or vector construct into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities and then divided by the luciferase activities of Gal4-MEF2C-WT or Gal4-MEF2C-K391R, respectively, to show the fold differences. (C) Gal4, Gal4-MEF2C-WT, or Gal4-MEF2C-K391R construct was co-transfected with LexA×8-GAL4×5-luciferase reporter, pRL-tk reporter, and LexA-VP16 construct into HeLa cells. Firefly luciferase activities were measured and normalized for transfection efficiency by using Renilla luciferase activities.