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Figure 1 | BMC Biochemistry

Figure 1

From: Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity

Figure 1

MEF2 proteins are sumoylated. (A) The 35S-labeled MEF2C protein obtained through in vitro transcription and translation was incubated with SUMO reaction mixtures and analyzed by SDS-PAGE followed by autoradiography. (B) Sequence alignment of the MEF2 family of proteins. The lysine residue of a sumoylation consensus motif and a serine residue that is phosphorylated are shown in bold. (C) The 35S-labeled wild-type (WT) and K391R mutant of MEF2C were incubated with SUMO reaction mixtures and analyzed by SDS-PAGE followed by autoradiography. (D) HeLa cells were transfected with the indicated plasmids. Myc-MEF2C was immunoprecipitated with anti-Myc. Cell lysates and Myc IP were resolved by SDS-PAGE and blotted with anti-Myc or anti-GFP. (E) HeLa cells were transfected with the indicated plasmids. Cell lysates were resolved by SDS-PAGE and blotted with anti-Myc. (F) HeLa cells were transfected with the indicated plasmids. Cell lysates were resolved by SDS-PAGE and blotted with anti-Myc.

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