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Figure 8 | BMC Biochemistry

Figure 8

From: Loss of NAD(H) from swollen yeast mitochondria

Figure 8

Nucleotide profile of swollen and unswollen mitochondria. The medium contained 0.6 M mannitol, 10 mM HEPES (TEA+), pH 7.20, and 15 μg/ml oligomycin. 2 mM ethanol was added to the mitochondria in the absence or presence of 10 mM KPi and incubated for 10 minutes. Mitochondria were then extracted for HPLC analysis. Mitochondria isolated from strains W303-1A or Yeast Foam were used as indicated. A numerical comparison of the nucleotide release from the swollen and unswollen mitochondria is shown in additional file 1: Table S1. Results are representative of 3 experiments from W303-1A and 2 experiments from Yeast Foam. Trends were conserved in the different experiments whereas exact values showed moderate variation.

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