Figure 7
Swelling stimulates NAD(H) release from mitochondria. Mitochondria were suspended in 0.6 M mannitol, 10 mM HEPES (TEA+), pH 7.20. 2 mM ethanol was added to the mitochondria in either the absence or presence of 10 mM KPi to allow or not allow swelling respectively. The mitochondria were then spun down. The supernatant was extracted, the NAD reduced, and the fluorescence assayed.