Figure 5
A membrane potential is maintained in the presence of decreased respiration. panel A, the medium contained 10 mM mannitol, 10 mM HEPES (TEA+), pH 7.20. Where indicated 0.6 M mannitol, 10 mM KPi or 500 μM NAD was present. 32 mM ethanol was added as shown. panel B, the conditions were the same as panel A except the membrane potential was monitored, 12 μM safranine O was present, and 4 μM FCCP was added where indicated.