Figure 4

NAD addition restores the respiratory rate of hypotonically swollen mitochondria. The medium contained 10 mM mannitol, 10 mM HEPES (TEA⁺), pH 7.20 and 0.1 mM decavanadate (Na⁺). 0.6 M mannitol was present where indicated. 0.5 mM NAD was added where indicated by an arrow or present from the beginning where indicated by t = 0. 32 mM ethanol was added at either 45 seconds or 7 minutes as indicated. inset, the conditions were the same as in the figure. The NAD concentration present was varied. The extents of oxygen consumption at 4 min. were compared.