Figure 2

The decreased respiratory rate does not occur when oxidizing NAD-linked substrates in a sucrose medium. panel A, the medium contained 0.6 M sucrose, 10 mM HEPES (TEA⁺), pH 7.20. Where indicated 10 mM KP_i was present. 4 mM ethanol or a combination of 4 mM pyruvate (Na⁺), 4 mM malate (K⁺) and 4 mM glutamate (K⁺) were added where indicated. panel B, the medium was the same as panel A except 32 mM ethanol was added instead of 4 mM ethanol.