Table 1 Summary of the purification protocol of the 1,3-1,4-β-glucanase produced by Rhizopus microsporus var. microsporus.
Steps | Total Protein(mg) | Total Activity(U) | Specific activity(U.mg-1) | Purification (-fold) | Yield(%) |
|---|---|---|---|---|---|
Concentrated crude extract | 1.574 | 0.228 | 0.197 | 1 | 100.000 |
Sephacryl S-100 eluate | 0.235 | 0.227 | 0.966 | 4.915 | 99.561 |
SP – Sepharose eluate | 0.021 | 0.262 | 12.596 | 55.529 | 114.912 |