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Figure 4 | BMC Biochemistry

Figure 4

From: Limited proteolysis of human histone deacetylase 1

Figure 4

Limited proteolysis of HDAC1 S421A and HDAC1 S423A mutants. Immunoprecipitated HDAC1 S421A (A) and HDAC1 S423A (B) mutants were incubated with increasing concentrations of trypsin (see Figure 1), separated by SDS-PAGE, and visualized with anti-Flag antibody. The faster migrating proteins present in the absence of trypsin (0X) are derived from the anti-Flag-bound solid phase used for immunoprecipitation [see Additional File 3]. (C) A graph showing percentage of full-length HDAC1 S421A (green) and HDAC1 S423A (blue) mutants remaining after exposure to different concentrations of trypsin. The data with wild type HDAC1-F (black- Figure 1) and HDAC1 S421A/S423A (red- Figure 2) proteins are also included for comparison. The curves were generated by least squares fit to a single exponential.

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