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Figure 5 | BMC Biochemistry

Figure 5

From: The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts

Figure 5

Xenopus Pol ε holoenzyme interacts directly with xGINS, xCdc45p, and xCut5p in vitro. (A) Flag-tagged r-xPol ε holoenzyme was incubated with xGINS [29] for 1 h at 4°C and immunoprecipitated with anti-Flag antibody. The immunoprecipitates were analyzed by SDS-PAGE followed by Western blotting. IP; immunoprecipitates, SUP; supernatant. (B) Increasing concentrations of xCut5p were incubated with xGINS and Flag-tagged xCdc45 [30] or Flag-tagged BAP for 1 h at 4°C and immunoprecipitated as in (A). (C) r-xPol ε holoenzyme was pre-incubated with either Flag-tagged xGINS or Flag-tagged xCdc45p prior to addition of the indicated protein(s). Reactions were analyzed as in (A). (D) A model for the interaction between r-xPol ε and replication accessory proteins (xGINS, xCdc45, and xCut5).

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BMC Biochemistry

ISSN: 1471-2091

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