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Figure 4 | BMC Biochemistry

Figure 4

From: The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts

Figure 4

DNA replication activity in Pol ε-depleted Xenopus egg extracts. (A) The same number of wild type-, mutant or partial r-xPol ε complex molecule, which has been estimated based on the result of Fig. 2, was added to xPol ε-depleted egg extracts as indicated (see text for detailed description of mutants and partial complexes). DNA replication was initiated by the addition of sperm chromatin and the rate and extent of DNA synthesis was measured by neutral agarose gel electrophoresis followed by autoradiography [22]. The origin (well) is and DNA size markers are indicated on the right. (B) Radioactive material, which migrated slower than the 23-kb marker DNA (shown by an arrow in (A)), was considered to be fully replicated product and quantified by a scintillation counter.

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