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Figure 3 | BMC Biochemistry

Figure 3

From: The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts

Figure 3

Interactions between histidine-tagged p260 (H-p260) or p60 (H-p60) and other subunits of Pol ε. Interactions between histidine-tagged p260 (H-p260) or p60 (H-p60) and other subunits of Pol ε were investigated using a pull down assay with Ni2+ -chelating beads and crude extracts prepared from insect cells expressing the indicated subunits of xPol ε. Cell lysates containing H-p260 or H-p60 (lane 1) and bound proteins (lane 2) were subjected to SDS-PAGE, followed by immunoblotting with the indicated antibodies. Beads without Ni2+ were also used as a control (lane 3).

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