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Figure 2 | BMC Biochemistry

Figure 2

From: The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts

Figure 2

Purified xPol ε holoenzyme from insect cells. Wild type and mutant FLAG-tagged p260 was co-expressed with p60, p17 and p12 in insect cells and Pol ε complexes were purified by DEAE Sepharose and anti-FLAG antibody chromatography. Fractions were eluted from the antibody affinity column, pooled and subjected to SDS-PAGE followed by CBB staining (Left) and immunoblotting (Right). Native Xenopus Pol ε (native) holoenzyme was purified from Xenopus egg extracts as described previously [22]. Fractions shown are rPol ε, rDN Pol ε, rΔcat Pol ε, and rF260 (see text). rF260 is FLAG-tagged p260.

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