HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1

Table 2 Comparison of rHSulf-2 requirements for mobilization of heparin-bound ligands

Ligand	Concentration required for 50% release of heparin-bound ligand
	rHSulf-2 (μg/mL)
VEGF	0.34
FGF-1	0.36
FGF-2	>4.0
CXCL12 (SDF-1)	0.44
CCL21 (SLC)	3.1
CXCL8 (IL-8)	3.4
CXCL10 (IP-10)^a	2.0

^a K_d for heparin-BSA was 62 nM. The fixed concentration of ligand tested in the assay was 50 nM.

ISSN: 1471-2091