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Table 1 Estimated dissociation constants for the interaction of ligands with immobilized heparin-BSA and heparan sulfate (HS)-BSA

From: HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1

Ligand

Kd value (nM)

 

Heparin-BSA

HS-BSA

VEGF

12.5

>200

FGF-1

9.2

26

FGF-2

8.6

N.D.

CXCL12 (SDF-1)

75

N.D.

CCL21 (SLC)

5.5

N.D.

CXCL8 (IL8)

>180

N.D.

  1. Each Kd value was estimated from the concentration of ligand needed for half-maximal saturation. In the cases of IL-8 binding to heparin-BSA and VEGF binding to HS-BSA, saturation was not observed at the highest concentration tested.
  2. N.D., not determined.
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BMC Biochemistry

ISSN: 1471-2091

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