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Table 1 Estimated dissociation constants for the interaction of ligands with immobilized heparin-BSA and heparan sulfate (HS)-BSA

From: HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1

Ligand Kd value (nM)
  Heparin-BSA HS-BSA
VEGF 12.5 >200
FGF-1 9.2 26
FGF-2 8.6 N.D.
CXCL12 (SDF-1) 75 N.D.
CCL21 (SLC) 5.5 N.D.
CXCL8 (IL8) >180 N.D.
  1. Each Kd value was estimated from the concentration of ligand needed for half-maximal saturation. In the cases of IL-8 binding to heparin-BSA and VEGF binding to HS-BSA, saturation was not observed at the highest concentration tested.
  2. N.D., not determined.