The effect of Sulf-2 on FGF-1 binding to heparan sulfate-BSA. FGF-1 binding to heparan sulfate-BSA was measured after the following treatments of the conjugate: no treatment; MCF-7 CM; rHSulf-2; rΔCCHSulf-2; or a mixture of heparinases. Values represent the means ± S.D. of three separate determinations. Statistical analysis was carried out using Student's t test. **, p < 0.01.