Skip to main content
Figure 1 | BMC Biochemistry

Figure 1

From: Influence of simulated microgravity on the activation of the small GTPase Rho involved in cytoskeletal formation – molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor

Figure 1

Effect of altered gravity on actin stress fiber formation in BBME cells. Panels A and B indicate the control and clinorotated cells, respectively. Clinorotation (B) results in the disorganization of actin stress fibers. BBME cells were cultured as described in the Methods section. After 72 h, the cells were fixed and stained with rhodamine-phalloidin and anti-paxillin antibody in combination with Alexa Fluor 488 to detect the actin stress fibers and paxillins, respectively. The cells were observed using a fluorescence microscope at ×400 magnification. The panels in the figures represent the best estimate of a typical field of cells under each condition. Scale bar = 10 μm.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us