(A) Further purification of APN fractions (fractions 19–21) from anion-exchange chromatography of An. quadrimaculatus BBMV by size-exclusion chromatography. A single peak was eluted at 75 ml elution volume, corresponding to 100 kDa. (B) SDS-PAGE of purified APN (APNAnq 100) obtained in (A) above. The estimated sizes of the protein bands are indicated on both sides of the gel in kDa.