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Table 3 IC50 calcium displacement values for various peptides/proteins. The molecular weights and the IC50-values (concentration of the peptides/proteins necessary to displace 50% of the calcium adsorbed to LPSF515 monolayers) are shown. Bathing solution: 12.5 μM calcium doped with radioactive 45Ca2+ resulting in a relative β-activity of 250 Bq/ml, buffered with 5 mM HEPES, and adjusted to pH7; the LPSF515 concentration was 9.6 nmol (160 nM).

From: Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins

Peptide/Protein

MW

IC50/μM

rCAP18

3801

0.22

lactoferrin

78922

0.12

rBPI21

21063

0.16

PMB

1200

0.84

PMBN

963

0.76

lactalbumin

66000

1

lysozyme

14400

6.9

rHSA

66000

14

hemoglobin

64000

32