Table 1 Properties of lipopolysaccharides and phospholipids used in this study. Given are the biochemical specifications of all lipids used in this study, the net charge calculated from mass spectrometric data, the results obtained from calcium adsorption experiments, given as the maximum β-intensity I max calculated according to Eq. 3, the electrophoretic mobilities μ, and the ζ-potential values. (n.d. – not determined, * – the values for phosphatidylcholine and phosphatidylglycerol could only be estimated)
Lipopolysaccharid | ||||||
|---|---|---|---|---|---|---|
Species | Strain | LPS chemotype | calculated net charge/e0 | I max /cps | μ/10-8 m2 V-1 s-1 | ζ-potential/mV |
S. minnesota | R595 | Re | - 3.6 | 265.8 ± 65.3 | -2.13 ± 0.13 | -27.2 ± 1.7 |
|  | R4 | Rd2 | - 4.0 | 345.3 ± 85.2 | -3.90 ± 0,63 | -49.9 ± 8.1 |
|  | R7 | Rd1 | - 4.1 | 307.9 ± 41.3 | -4.35 ± 0.06 | -55.7 ± 0.8 |
|  | Rz | Rd1 | - 5.3 | 537.6 ± 69.2 | -3.09 ± 0.28 | -39.5 ± 3.6 |
|  | R5 | Rc | - 4.1 | 307.9 ± 37.8 | -2.65 ± 0.45 | -33.9 ± 5.7 |
|  | R345 | Rb2 | - 4.8 | 545.9 ± 105.0 | -1.04 ± 0.41 | -13.3 ± 5.2 |
|  | R60 | Ra | - 6.0 | 480.8 ± 91.6 | -2.63 ± 0.03 | -33.7 ± 0.4 |
E. coli | F515 | Re | - 4.0 | 451.6 ± 119.7 | -3.27 ± 0.12 | -41.9 ± 1.5 |
P. mirabilis | R45 | Re | - 3.0 | 265.8 ± 65.3 | -4.21 ± 0.09 | -53.9 ± 1.2 |
Phospholipids | ||||||
|  |  |  | calculated net charge/e 0 | I max /cps | μ/10 -8 m 2 V -1 s -1 | ζ-potential/mV |
phosphatidylglycerol | - 1.0 | ~60 ± 44.4* | -5.76 ± 0.23 | -73.7 ± 2.9 | ||
phosphatidylserine | - 1.0 | n.d. | -5.09 ± 1.37 | -65.1 ± 17.5 | ||
diphosphatidylglycerol | - 2.0 | 182.4 ± 61.8 | -5.83 ± 0.66 | -74.6 ± 8.4 | ||
phosphatidylcholine | 0 | ~0 ± 23.4* | -0.43 ± 0.10 | -5.5 ± 1.3 | ||
phosphatidylethanolamine | 0 | n.d. | -1.20 ± 0.03 | -15.4 ± 0.4 | ||