Figure 7

Displacement of calcium from LPS F515 monolayers by polymyxin B nonapeptide and lysozyme. Relative change of the β-intensity in dependence on peptide/protein concentration after addition of the nonapeptide of polymyxin B () or lysozyme () into the subphase underneath LPSF515 monolayers. Bathing solution: 12.5 μM Ca²⁺ doped with radioactive ⁴⁵Ca²⁺ resulting in a relative β-activity of 250 Bq/ml, buffered with 5 mM HEPES, and adjusted to pH7 and the LPS F515 concentration was 9.6 nmol (160 nM).