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Figure 7 | BMC Biochemistry

Figure 7

From: Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins

Figure 7

Displacement of calcium from LPS F515 monolayers by polymyxin B nonapeptide and lysozyme. Relative change of the β-intensity in dependence on peptide/protein concentration after addition of the nonapeptide of polymyxin B () or lysozyme () into the subphase underneath LPSF515 monolayers. Bathing solution: 12.5 μM Ca2+ doped with radioactive 45Ca2+ resulting in a relative β-activity of 250 Bq/ml, buffered with 5 mM HEPES, and adjusted to pH7 and the LPS F515 concentration was 9.6 nmol (160 nM).

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