Aquaporin-11: A channel protein lacking apparent transport function expressed in brain

BMC Biochemistry

Table 2 AQP11 injected oocytes show no increase in water, glycerol, or urea transport at pH 4.5, 6.5, or 8.5, and no increase in ion conductance at pH 4.5 or 8.5.

	Water Transport	Glycerol Transport	Urea Transport	Ion Conductance
pH 4.5
control	8.87 ± 3.76			2.2 ± 0.2
hAQP1	234.06 ± 23.52	-4.41 ± 1.91	4.77 ± 4.42
myc-hAQP11	8.57 ± 4.19	-0.94 ± 1.63	4.23 ± 4.82	1.7 ± 0.2
pH 6
control	19.13 ± 8.2	0.63 ± 1.83	3.61 ± 5.2
hAQP1	209.22 ± 85.63
myc-hAQP11	27.09 ± 29.76	0.24 ± 2.57	1.17 ± 4.23
pH 8.5
control	31.23 ± 11.83			4.7 ± 1.4
rAQP4	196.16 ± 56.43	9.26 ± 3.24	-3.65 ± 5.11
hAQP11	33.92 ± 37.23			5.7 ± 2.8
rAQP11				5.1 ± 1.1
rAQP6				23 ± 2.6
25 ng hAQP11	20.85 ± 6.69	4.21 ± 5.18	-0.32 ± 1.97

Xenopus oocytes were injected with 50 nl water (control), or with 5 ng human AQP1 (hAQP1), rat AQP2 (rAQP2), rat AQP4 M23 (rAQP4), rat AQP6 (rAQP6), rat AQP11 (rAQP11), human AQP11 (hAQP11), myc-tagged human AQP11 (myc-hAQP11), or with 25 ng human AQP11 cRNA and tested for water, glycerol, or urea transport or ion conductance at indicated pH. Units for water transport are μm/sec, for glycerol and urea transport 10^-6 cm/sec, for ion conductance μA/V. Data represent mean ± SD for 6–10 oocytes, except for ion conductance which is mean ± SE for 3–5 oocytes.

ISSN: 1471-2091