Aquaporin-11: A channel protein lacking apparent transport function expressed in brain

BMC Biochemistry

Table 1 AQP11 injected oocytes show no increase in water, glycerol or urea transport, and no increase in ion conductance at pH 7.5.

	Water Transport	Glycerol Transport	Urea Transport	Ion Conductance
control	16.88 ± 6.65	1.53 ± 2.31	-2.04 ± 7.05	2.8 ± 0.6
hAQP1	263.92 ± 42.22
myc-hAQP11	15.32 ± 5.64	1.67 ± 3.25	0.96 ± 2.78	1.9 ± 0.1
rAQP9		21.12 ± 7.57	16.85 ± 9.33
control Hg		2.18 ± 3.76	0.74 ± 1.91
rAQP9 Hg		-1.44 ± 1.06	0.53 ± 2.11
myc-hAQP11 Hg		-0.52 ± 1.12	2.51 ± 2.88
control	20.63 ± 9.94			3.5 ± 1.1
rAQP2	142.91 ± 39.21
25 ng hAQP11	11.89 ± 2.32
hAQP11	11.26 ± 4.77	0.79 ± 1.27	0.03 ± 0.24	5.3 ± 2.5
rAQP9		11.84 ± 4.69	11.14 ± 5.17
control Hg	28.39 ± 13.58
rAQP2 Hg	54.11 ± 21.39
hAQP11 Hg	29.62 ± 23.90
rAQP11				4.4 ± 0.7
rAQP6				10.0 ± 3.4
control	10.86 ± 14.23	5.74 ± 6.61	-0.45 ± 2.21
hAQP1	196.61 ± 24.52
rAQP11	17.64 ± 10.89	5.91 ± 8.24	0.005 ± 1.14
rAQP9			3.11 ± 2.64

Xenopus oocytes were injected with 50 nl water (control), or with 5 ng human AQP1 (hAQP1), rat AQP2 (rAQP2), rat AQP9 (rAQP9), human AQP11 (hAQP11), myc-tagged human AQP11 (myc-hAQP11), or with 25 ng human AQP11 cRNA and tested for water, glycerol, urea, and ion transport. Some oocytes were pre-incubated for 5 minutes with 0.1 mM HgCl₂ (Hg). Units for water transport are μm/sec, for glycerol and urea transport 10^-6 cm/sec, and for ion conductance μA/V. Data represent mean ± SD for 6–10 oocytes, except for ion conductance which is mean ± SE for 3–5 oocytes.

ISSN: 1471-2091