Figure 5

hARD2 interacts with NATH and HIF-1α. (A)HEK293 cells, transfected with plasmids encoding hARD2-V5 or lacZ-V5 as a negative control, were harvested and the lysates were immunoprecipitated (IP) with the anti-V5 antibody. The immunoprecipitates were analysed by SDS-PAGE and Western Blotting. The membrane was incubated with anti-NATH. The amount of lysate loaded on the gel represents approx. 10 % of the material used in the immunoprecipitation reaction. (B) MCF-7 cells were cotransfected with plasmids encoding HA-HIF-1α and X-press-hARD2 or X-press-lacZ as a negative control. After 48 hours the cells were collected and processed as (A). The membrane was incubated with anti-HA.