Table 1 Production ratio of mutant in baculovirus compared to wild type enzyme. Reference is the wild type Dm AChE which was produced at 52 nanomoles per liter of culture. *: significant difference, n: number of batches analyzed
Relative production | ||||||
|---|---|---|---|---|---|---|
Mutant code | Mutated amino-acids | Grade (MODIP) | distance (Cβ) in tertiary structure (Å) | n | mean | Standard error |
m1 | R24/A169 | B | 3.65 | 5 | 1.43 | 0.71 |
m2 | I327/D375 | C | 3.77 | 20 | 1.06 | 0.73 |
m3 | L354/A456 | C | 3.56 | 19 | 0.22 * | 0.26 |
m4 | T369/M476 | A | 3.62 | 15 | 0.04 * | 0.07 |
m5 | L388/Q427 | C | 3.70 | 16 | 0.96 | 0.63 |
m6 | A452/S533 | B | 3.91 | 7 | 0.73 | 0.45 |
m7 | T464/S543 | D | 3.96 | 8 | 0.77 | 0.47 |