The role of aspartate and aspartate transport in the pathway of prostate citrate production. Aspartate via mAAT provides the four-carbon source of oxalacetate that condenses with acetyl coA for citrate synthesis. The oxidation of citrate via the Krebs cycle is prevented by the inhibition of m-aconitase by zinc. The utilized aspartate is replenished by the transport of aspartate from interstitial fluid by the EAAC1 aspartate transporter. Note the extremely high concentration of citrate in prostatic fluid secretion that requires a substantial and continuous supply of aspartate. mAAT = mitochondrial aspartate aminotransferase; GDH = glutamic dehydrogenase; CS = citrate synthase; ACON = mitochondrial aconitase; PDH = pyruvate dehydrogenase.