Effect of testosterone on EAAC1. A. Freshly prepared rat ventral VP epithelial cells were incubated for 3 hours in HBSS medium that contained 1 nM testosterone or vehicle (control). Loading gel (not shown) showed no loading differences between the control and testosterone samples. B. Effects of prolactin and testosterone on high-affinity aspartate transport of rat ventral prostate. The cells were harvested from donor animals that were castrated and bromocryptine-treated to reduce endogenous levels of hormones. CHX= 30 uM cyclohexamide; PRL= 1 nM; TEST= 10 nM. * P < 0.05 vs control.