Figure 2

Loss of Csn5 results in a decrease in F-box protein levels. (A) Depletion of Csn5 results in a decrease in F-box protein levels. Cells were treated with doxycycline for eight days followed by lysis and SDS-PAGE/western blot analysis with antibodies toward the proteins indicated. (B) Depletion of Csn5 does not affect F-box protein mRNA levels. Cells were treated with doxycycline for eight days and analyzed by RT-PCT using oligos specific toward the mRNAs indicated.