Figure 6

The initiator element in the adenoviral major late promoter luciferase construct (pGLMLP Luc) increases the response to Myc but does not allow further stimulation by TBP. Compared with the reporter containing a mutant InR (pGLMLP dINR Luc), which displays a highly synergistic stimulation by Myc and TBP, the wild-type pGLMLP Luc construct briskly responds to Myc independent of increasing amounts of TBP (μg indicated on the abscissa). Bars are shown as averages with standard deviation (n = 10). Transfections were performed using NIH 3T3 cells.