Figure 5
From: Stimulation of Myc transactivation by the TATA binding protein in promoter-reporter assays
Myc DNA binding sites in the LDHA promoter construct (LDHLuc) is required for TBP stimulation of Myc-mediated transactivation. Wild-type LDHLuc or a mutant promoter construct (LDHDMLuc), which has both Myc E-boxes mutated from 5'-CACGTG-3' to 5'-CCCGGG-3', were cotransfected with a constant amount of MLV-LTR driven wild-type c-myc plasmid and increasing amounts of TBP (μg indicated on the abscissa). Bars are shown as averages with standard deviation (n = 6). Transfections were performed using NIH 3T3 cells.