Figure 4
From: Stimulation of Myc transactivation by the TATA binding protein in promoter-reporter assays
Activation of the lactate dehydrogenase A promoter construct (LDHLuc) by Myc is increased by TBP. Activator alone: cotransfection with LDHLuc and MLV-LTR driven Myc expression vectors: MLV, empty expression vector; Myc, wild-type Myc; dHLH, helix-loop-helix deletion mutant D371-412; W135E, substitution mutant with glutamate replacing tryptophan 135. Increasing amounts of TBP (μg indicated on the abscissa) was cotransfected with Myc and reporter constructs. Bars are shown as averages with standard deviation (n = 10). Transfections were performed using NIH 3T3 cells.