Figure 2

TBP does not stimulate initiator driven luciferase reporter G5INRLuc that contains five GAL4 binding sites. Either G5INRLuc or G5TATALuc (see Fig. 1) was cotransfected into CHO cells with GM1-262 alone or with increasing amounts of TBP (μg indicated on the abscissa). For comparison, data points for G5TATALuc are the same as those shown in Fig. 1. Note that TBP does not stimulate GAL4Myc transactivation of G5INRLuc; there is a slight inhibition by TBP. Bars are shown as averages with standard deviation (n = 4).