Figure 1
From: Stimulation of Myc transactivation by the TATA binding protein in promoter-reporter assays
TBP stimulates GAL4-Myc transactivation. The reporter G5TATALuc contains five GAL4 binding sites followed by a minimal TATA box and the luciferase cDNA. Activator alone: the reporter was cotransfected with GAL4(1-147) DNA binding domain (GALO), GAL4 fused to Myc residues 1-262 (GM1-262), GM1-262 with residues 106–143 deleted (GM1-262D106), or GM1-262 with a substitution of tryptophan 135 to glutamate (GMW135E) alone. TBP at increasing input plasmid amounts (μg indicated on the abscissa) was cotransfected into CHO cells with the reporter alone (TBP) or with the indicated GAL4 plasmids. Bars are shown as averages with standard deviation (n = 10).