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Figure 3 | BMC Biochemistry

Figure 3

From: A novel mass spectrometry-based assay for GSK-3β activity

Figure 3

2B-Sp m/z ratio shifts in response to varying amounts of lithium chloride, and sodium chloride. GSK-3β specificity for the 2B-Sp target peptide was examined in a kinase assay with lithium chloride (LiCl, green bars, n = 3) a well-known inhibitor of GSK-3β, and sodium chloride (NaCl, purple bars, n = 3), a salt with no known GSK-3β inhibitory characteristics. Increasing amounts of LiCl (0–50 mM), or NaCl (0–50 mM) were added to kinase assays containing 125 ng of 2B-Sp peptide, and 6.25 ng of recombinant GSK-3β. M/z ratios were subsequently analyzed using SELDI-TOF-MS. Pooled data for LiCl assays are represented as mean +/- SEM values. NaCl assays are represented as mean values.

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