Figure 2

A dose-dependent shift in 2B-Sp m/z ratio in response to increasing amounts of recombinant GSK-3β. 125 ng of 2B-Sp was added to kinase assays with increasing amounts of recombinant GSK-3β (0 – 6.25 ng). (A) Raw data is presented here as a SELDI-TOF-MS m/z read-out. (B) Enzyme activity was measured as percent 2B-Sp phosphorylation, and was determined through the ratio calculation of the 2143.2 Da. peak intensity to the total signal intensity values of all peptide present in the sample (2063.2 Da. and 2143.2 Da. peaks combined). Pooled data are plotted as mean values +/- SEM (n = 3) at each data point.