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Table 1 Catalytic parameters of Csk and Asn319Ser mutant

From: Characterization of the interactions between the active site of a protein tyrosine kinase and a divalent metal activator

Parametera

Wt Csk

Asn319Ser

kcat-ATP (min-1)

160 ± 10

0.01 ± 0.007

Km-ATP (μM)

140 ± 12

150 ± 25

kcat-polyE4Y (min-1)

82 ± 12

0.01 ± 0.001

Km-polyE4Y (μg ml-1)

156 ± 30

220 ± 48

kcat-kdSrc (min-1)

109 ± 3

0.01 ± 0.001

Km-kdSrc (μM)

6.4 ± 0.1

3.4 ± 0.1

  1. aAll assays were performed at least three times. The standard errors were calculated from three most consistent assays.
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BMC Biochemistry

ISSN: 1471-2091

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