Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers

Table 3 Isotopolog compositions of amino acids from S. frugiperda cells grown in a mixture of [U-¹³C₆]glucose and natural abundance glucose.

Compound	Position	¹³ Chemical Shift (δ, ppm)	Coupling Constants J_CC (Hz)	%¹³C^a	¹³C¹³C^b
Alanine (4)	1	175.7	n.d.	n.d.	n.d.
	2	51.4	59.6 (1), 34.9 (3)	1.8	3.8 (3), 22.2 (1, 3)
	3	18.4	35.1 (2)	1.7	26.5 (2)
Aspartate (5)	1	182.9	n.d.	n.d.	n.d.
	2	61.3	60.4 (1)	1.8	7.4 (1)
	3	45.9	55.3 (4)	1.9	7.2 (4)
	4	185.1	55.7 (3)	1.8	7.6 (3)
Glutamate (6)	1	176.1		1.3
	2	56.2		1.0
	3	27.9		1.1
	4	32.5	55.1 (5)	1.8	17.1 (5)
	5	179.6	55.1 (4)	1.7	17.2 (4)

^aabsolute ¹³C abundance.
^brelative fraction of satellite pairs in the global ¹³C NMR intensity of the index carbon atom. The coupling partners of the index carbon atom are indicated in parentheses.
n.d., not determined due to poor signal quality.

ISSN: 1471-2091